valin A was shown to agglutinate normal lymphocytes, lym phoma cells, and leukemic cells from chronic lymphocytic leukemia and from acute myelocytic and lymphocytic leuke

With a newly developed turbidometric method, concana valin A was shown to agglutinate normal lymphocytes, lym phoma cells, and leukemic cells from chronic lymphocytic leukemia and from acute myelocytic and lymphocytic leuke mia. However, there was a marked difference in the kinetics of this agglutination process. Leukemic blast cells and cells from a patient with convoluted lymphoma agglutinated poorly in this system. Conversely, the degree of agglutina tion for chronic lymphocytic leukemia cells was greater than that for the blast cells and also slightly greater than that for normal lymphocytes. Cultured cells from a Burkitt's lymphoma (Raji) and from a patient with poorly differen tiated lymphoma agglutinated very rapidly with concana valin A. Prior incubation of all cell types with neuraminidase markedly enhanced the agglutination process similar to that of trypsinization. Thus, these studies illustrate the useful nessof this method in quantitating the kinetics of agglutina tion of various human neoplastic cell types by concanavalin A.